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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from <t>LabChart.</t> Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.
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Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from LabChart. Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.

Journal: Journal of Molecular and Cellular Cardiology Plus

Article Title: Independent and synergistic roles of MEK-ERK1/2 and PKC pathways in regulating functional changes in vascular tissue following flow cessation

doi: 10.1016/j.jmccpl.2025.100300

Figure Lengend Snippet: Comparison of fresh BAs and BAs after 48 h of incubation in organ culture regarding ET B receptors: (a) Immunofluorescence of ET B receptors of the smooth muscle cell layer in fresh rat BAs and BAs after organ culture (48H) (b) Quantification of ET B immunofluorescence, compared with Student's t-test (c) Contraction (mN) to 60 mM K + , compared with Student's t-test (d) Log concentration-response curves in response to S6c, and values compared by repeated measures two-way ANOVA, with Sidak's post-test (e) Representative sample traces from LabChart. Effects of S6c Data show the contractile responses in rat BAs to cumulative contractions of sarafotoxin (S6c). Arrows indicate the times at which different concentrations were applied (Biological replicate; n = 5–9). * = p < 0.05.

Article Snippet: The myographs were connected to a PowerLab Unit and responses were sampled in LabChart™ (ADInstruments, UK).

Techniques: Comparison, Incubation, Organ Culture, Immunofluorescence, Concentration Assay

Comparison of the effects of the MEK inhibitor Trametinib and PKC inhibitor, RO-317549 to the S6C-mediated contraction of BAs after a 48-h OC . (a) Log concentration-response curves in response to S6c, following incubation with 10 −10 –10 −6 M Trametinib, (b) E max(S6c) responses of BAs incubated with different concentrations of Trametinib, (c) Log concentration-response curves in response to S6c, following incubation with 10 −8 –10 −5 M RO-317549 , (d) E max(S6c) responses of BAs incubated with different concentrations of RO-317549, (e) E max(S6c) responses of BAs incubated with different concentrations of Trametinib, and combination of RO-317549 10 −6 M with different concentrations of Trametinib. The Trametinib curve is the same as shown in panel b. The HillSlope was fixed at −1.727, (f) Representative Sample Traces from LabChart. CTRL refers to BAs incubated for a 48-h in OC with DMSO/Vehicle. Data are shown as mean ± SEM (Biological replicate; n = 5–18). Values were compared with a Student's t -test with a multiple correction (Holm-Sidak). * = p < 0.05.

Journal: Journal of Molecular and Cellular Cardiology Plus

Article Title: Independent and synergistic roles of MEK-ERK1/2 and PKC pathways in regulating functional changes in vascular tissue following flow cessation

doi: 10.1016/j.jmccpl.2025.100300

Figure Lengend Snippet: Comparison of the effects of the MEK inhibitor Trametinib and PKC inhibitor, RO-317549 to the S6C-mediated contraction of BAs after a 48-h OC . (a) Log concentration-response curves in response to S6c, following incubation with 10 −10 –10 −6 M Trametinib, (b) E max(S6c) responses of BAs incubated with different concentrations of Trametinib, (c) Log concentration-response curves in response to S6c, following incubation with 10 −8 –10 −5 M RO-317549 , (d) E max(S6c) responses of BAs incubated with different concentrations of RO-317549, (e) E max(S6c) responses of BAs incubated with different concentrations of Trametinib, and combination of RO-317549 10 −6 M with different concentrations of Trametinib. The Trametinib curve is the same as shown in panel b. The HillSlope was fixed at −1.727, (f) Representative Sample Traces from LabChart. CTRL refers to BAs incubated for a 48-h in OC with DMSO/Vehicle. Data are shown as mean ± SEM (Biological replicate; n = 5–18). Values were compared with a Student's t -test with a multiple correction (Holm-Sidak). * = p < 0.05.

Article Snippet: The myographs were connected to a PowerLab Unit and responses were sampled in LabChart™ (ADInstruments, UK).

Techniques: Comparison, Concentration Assay, Incubation